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(A) Schematic illustration of the experimental design. Syrian hamsters were infected intraperitoneally with OROV (5.4 x 10 5 PFU) and treated with favipiravir (100 or 600 mg/kg/day) or ribavirin (40 or 100 mg/kg/day) starting 1 hour before infection, twice daily at 8-hour intervals until 6 days post-infection (dpi). The dashed grey box indicates the treatment window. Animals were monitored for (B) survival and (C) body weight changes until 26 dpi (n = 6 per group). (D-G) Quantification of viral <t>RNA</t> <t>by</t> <t>RT-qPCR</t> in the (D) serum, (E) brain, (F) liver and (G) spleen collected at the indicated time points. Copy numbers were calculated based on standard curves. (H - K) Infectious viral titers determined by plaque assay in the (H) serum, (I) brain, (J) liver and (K) spleen at the indicated time points. (D-K) Vehicle-treated animals succumbed to infection after 2 dpi; therefore, samples were only available at 2 dpi for this group. N/A: not applicable. Dashed lines indicate the limit of detection. Bars represent geometric means ± geometric s.d. and dots represent individual animals (n = 4 per group) from a representative experiment. Statistical significance was assessed by Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 . Panel (A) was created in BioRender. Tabata, K. (2026) https://BioRender.com/jl0r5d6 .
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(A) Schematic illustration of the experimental design. Syrian hamsters were infected intraperitoneally with OROV (5.4 x 10 5 PFU) and treated with favipiravir (100 or 600 mg/kg/day) or ribavirin (40 or 100 mg/kg/day) starting 1 hour before infection, twice daily at 8-hour intervals until 6 days post-infection (dpi). The dashed grey box indicates the treatment window. Animals were monitored for (B) survival and (C) body weight changes until 26 dpi (n = 6 per group). (D-G) Quantification of viral <t>RNA</t> <t>by</t> <t>RT-qPCR</t> in the (D) serum, (E) brain, (F) liver and (G) spleen collected at the indicated time points. Copy numbers were calculated based on standard curves. (H - K) Infectious viral titers determined by plaque assay in the (H) serum, (I) brain, (J) liver and (K) spleen at the indicated time points. (D-K) Vehicle-treated animals succumbed to infection after 2 dpi; therefore, samples were only available at 2 dpi for this group. N/A: not applicable. Dashed lines indicate the limit of detection. Bars represent geometric means ± geometric s.d. and dots represent individual animals (n = 4 per group) from a representative experiment. Statistical significance was assessed by Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 . Panel (A) was created in BioRender. Tabata, K. (2026) https://BioRender.com/jl0r5d6 .
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(A) Schematic illustration of the experimental design. Syrian hamsters were infected intraperitoneally with OROV (5.4 x 10 5 PFU) and treated with favipiravir (100 or 600 mg/kg/day) or ribavirin (40 or 100 mg/kg/day) starting 1 hour before infection, twice daily at 8-hour intervals until 6 days post-infection (dpi). The dashed grey box indicates the treatment window. Animals were monitored for (B) survival and (C) body weight changes until 26 dpi (n = 6 per group). (D-G) Quantification of viral <t>RNA</t> <t>by</t> <t>RT-qPCR</t> in the (D) serum, (E) brain, (F) liver and (G) spleen collected at the indicated time points. Copy numbers were calculated based on standard curves. (H - K) Infectious viral titers determined by plaque assay in the (H) serum, (I) brain, (J) liver and (K) spleen at the indicated time points. (D-K) Vehicle-treated animals succumbed to infection after 2 dpi; therefore, samples were only available at 2 dpi for this group. N/A: not applicable. Dashed lines indicate the limit of detection. Bars represent geometric means ± geometric s.d. and dots represent individual animals (n = 4 per group) from a representative experiment. Statistical significance was assessed by Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 . Panel (A) was created in BioRender. Tabata, K. (2026) https://BioRender.com/jl0r5d6 .
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(A) Schematic illustration of the experimental design. Syrian hamsters were infected intraperitoneally with OROV (5.4 x 10 5 PFU) and treated with favipiravir (100 or 600 mg/kg/day) or ribavirin (40 or 100 mg/kg/day) starting 1 hour before infection, twice daily at 8-hour intervals until 6 days post-infection (dpi). The dashed grey box indicates the treatment window. Animals were monitored for (B) survival and (C) body weight changes until 26 dpi (n = 6 per group). (D-G) Quantification of viral <t>RNA</t> <t>by</t> <t>RT-qPCR</t> in the (D) serum, (E) brain, (F) liver and (G) spleen collected at the indicated time points. Copy numbers were calculated based on standard curves. (H - K) Infectious viral titers determined by plaque assay in the (H) serum, (I) brain, (J) liver and (K) spleen at the indicated time points. (D-K) Vehicle-treated animals succumbed to infection after 2 dpi; therefore, samples were only available at 2 dpi for this group. N/A: not applicable. Dashed lines indicate the limit of detection. Bars represent geometric means ± geometric s.d. and dots represent individual animals (n = 4 per group) from a representative experiment. Statistical significance was assessed by Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 . Panel (A) was created in BioRender. Tabata, K. (2026) https://BioRender.com/jl0r5d6 .
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(A) Schematic illustration of the experimental design. Syrian hamsters were infected intraperitoneally with OROV (5.4 x 10 5 PFU) and treated with favipiravir (100 or 600 mg/kg/day) or ribavirin (40 or 100 mg/kg/day) starting 1 hour before infection, twice daily at 8-hour intervals until 6 days post-infection (dpi). The dashed grey box indicates the treatment window. Animals were monitored for (B) survival and (C) body weight changes until 26 dpi (n = 6 per group). (D-G) Quantification of viral <t>RNA</t> <t>by</t> <t>RT-qPCR</t> in the (D) serum, (E) brain, (F) liver and (G) spleen collected at the indicated time points. Copy numbers were calculated based on standard curves. (H - K) Infectious viral titers determined by plaque assay in the (H) serum, (I) brain, (J) liver and (K) spleen at the indicated time points. (D-K) Vehicle-treated animals succumbed to infection after 2 dpi; therefore, samples were only available at 2 dpi for this group. N/A: not applicable. Dashed lines indicate the limit of detection. Bars represent geometric means ± geometric s.d. and dots represent individual animals (n = 4 per group) from a representative experiment. Statistical significance was assessed by Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 . Panel (A) was created in BioRender. Tabata, K. (2026) https://BioRender.com/jl0r5d6 .
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Image Search Results


Journal: iScience

Article Title: B cells in the retinal pigment epithelium change with aging and IgM availability

doi: 10.1016/j.isci.2026.117640

Figure Lengend Snippet:

Article Snippet: DNA/RNA shield , Zymo , Cat# R1100.

Techniques: Recombinant, Staining, Lysis, Enzyme-linked Immunosorbent Assay, Stem Cell, Qubit, Platinum, Sequencing, Bulk RNA Sequencing, Software

(A) Schematic illustration of the experimental design. Syrian hamsters were infected intraperitoneally with OROV (5.4 x 10 5 PFU) and treated with favipiravir (100 or 600 mg/kg/day) or ribavirin (40 or 100 mg/kg/day) starting 1 hour before infection, twice daily at 8-hour intervals until 6 days post-infection (dpi). The dashed grey box indicates the treatment window. Animals were monitored for (B) survival and (C) body weight changes until 26 dpi (n = 6 per group). (D-G) Quantification of viral RNA by RT-qPCR in the (D) serum, (E) brain, (F) liver and (G) spleen collected at the indicated time points. Copy numbers were calculated based on standard curves. (H - K) Infectious viral titers determined by plaque assay in the (H) serum, (I) brain, (J) liver and (K) spleen at the indicated time points. (D-K) Vehicle-treated animals succumbed to infection after 2 dpi; therefore, samples were only available at 2 dpi for this group. N/A: not applicable. Dashed lines indicate the limit of detection. Bars represent geometric means ± geometric s.d. and dots represent individual animals (n = 4 per group) from a representative experiment. Statistical significance was assessed by Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 . Panel (A) was created in BioRender. Tabata, K. (2026) https://BioRender.com/jl0r5d6 .

Journal: PLOS Pathogens

Article Title: Integrated in vivo and transcriptomic analyses of lethal Oropouche virus infection reveal suppression of pathogenic host responses by antiviral therapy

doi: 10.1371/journal.ppat.1014647

Figure Lengend Snippet: (A) Schematic illustration of the experimental design. Syrian hamsters were infected intraperitoneally with OROV (5.4 x 10 5 PFU) and treated with favipiravir (100 or 600 mg/kg/day) or ribavirin (40 or 100 mg/kg/day) starting 1 hour before infection, twice daily at 8-hour intervals until 6 days post-infection (dpi). The dashed grey box indicates the treatment window. Animals were monitored for (B) survival and (C) body weight changes until 26 dpi (n = 6 per group). (D-G) Quantification of viral RNA by RT-qPCR in the (D) serum, (E) brain, (F) liver and (G) spleen collected at the indicated time points. Copy numbers were calculated based on standard curves. (H - K) Infectious viral titers determined by plaque assay in the (H) serum, (I) brain, (J) liver and (K) spleen at the indicated time points. (D-K) Vehicle-treated animals succumbed to infection after 2 dpi; therefore, samples were only available at 2 dpi for this group. N/A: not applicable. Dashed lines indicate the limit of detection. Bars represent geometric means ± geometric s.d. and dots represent individual animals (n = 4 per group) from a representative experiment. Statistical significance was assessed by Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test: *p < 0.05, **p < 0.01 and ***p < 0.001 . Panel (A) was created in BioRender. Tabata, K. (2026) https://BioRender.com/jl0r5d6 .

Article Snippet: Tissues were homogenized either in PBS, for viral titration and RT-qPCR or RNA Shield (Zymo Research) for RNAseq.

Techniques: Infection, Quantitative RT-PCR, Plaque Assay, Comparison